Nucleus Basalis of Meynert as a target for deep brain stimulation

OverviewThis dataset comprises electrophysiology recordings from 9 B6 male mice, conducted during multiple sleep-wake cycles while animals were behaving freely in standard mouse home cages. Data set includes background recordings without stimulations (no_stm) and recordings made during bipolar stimulation on Nucleus Basalis of Meynert using 3 types of stimulation pattern: nested (stm_nested), random (stm_random) and tonic (stm_tomnic). For details see manuscript doi: https://doi.org/10.1101/2025.06.11.659014 archived at https://www.biorxiv.org/content/10.1101/2025.06.11.659014v1.

Data were acquired using Intan systems (RHS at 20 kHz for mice 2-9; RHD at 10 kHz for mouse 1 for no stimulation, then RHS at 20 kHz for all stimulation conditions), with onboard filtering from 0.5-5000 Hz. Channels include nucleus basalis (NB1, NB2), cortical regions (A: anterior insular cortex; V: visceral insular cortex; S: supplementary somatosensory; M: primary motor cortex), EEG, and cervical muscle EMG. Markers designating different states of vigilance (sws: slow wave sleep; rem: REM sleep; aw: active wakefulness; qw: quiet wakefulness) were created for each recording and stored as .txt files containing timestamps. Markers are representative of each state of vigilance, but do not cover all recorded data: e.g., intervals containing motion artifacts were avoided.

Ethical approval was obtained from University of Florey Animal Ethics Committee (21-071-UM). For queries, contact depositor.

1. Raw data

Acquisition DetailsRecordings span continuous sessions across sleep-wake transitions without stimulation unless specified in filenames (e.g., "no_stm" indicates no stimulation; see other records made with these animals). Signals contain local field potentials and multi-unit activity from 2 nucleus basalis electrodes, 3-4 cortical channels, EEG, and EMG per animal. Vigilance state markers align with raw traces for analysis of epochs.

File StructureData are stored as plain text (.txt) files, one per channel per recording session.

Filename convention: {mouse_id}_{condition}_{source}.txt (e.g., m1_no_stm_M.txt for mouse 1, no stimulation, primary motor cortex).

Data files contain voltage traces in columnar format (1 column per file).

Marker files: {mouse_id}_{condition}_{state}.txt (e.g., m1_no_stm_sws.txt).

Organized by mouse in zip archives containing recorded data for one animal in one conditions (e.g. m10_stm_nested.zip – mouse 10, nested stimulation).

2. Processed data summary

The supporting XLSX files contain processed data for the spike-field coherence (SFC) and power spectral density (PSD) analyses reported in the manuscript. All files are placed in the same archive titled "population_data_summary.zip".

Spike-Field Coherence Files: 8 files total: separate files for each of 4 vigilance states (sws, rem, aw, qw), with 2 files per state

NB cells to NB LFP: Files titled "Supporting_summary_NB_cells_to_NB_LFP_SF_significant_above_confidence_threshold_[state].xlsx".Column A: Frequencies, Hz.Column B: Row labels (cell number, mouse ID).All further columns: Binary SFC values (1 = coherence exceeds 95% Jackknife confidence interval over ≥3.57 Hz bandwidth as per Methods; 0 = below threshold) for each cell.

Cortical cells to NB LFP: Files titled "Supporting_summary_Cortical_cells_to_NB_LFP_SF_significant_above_confidence_threshold_[state].xlsx".Column A: Frequencies, Hz.Column B: Row labels (cell number, mouse ID, cortical area: M1/SS/Vi/Ai).All further columns: Binary SFC values (1 = coherence exceeds 95% Jackknife confidence interval over ≥3.57 Hz bandwidth as per Methods; 0 = below threshold), for each cortical cell.

Population PSD File: "Supporting_summary__stimulation_evoked_PSDs.xlsx" (sheets: SWS, REM, QW).

Column A: Stimulation pattern (nested, tonic 20 Hz, random).

Column B: Animal ID.

Column C: Cortical area (M1/SS/Vi/Ai).

Subsequent columns: Mean PSD integrals (μV²) for frequency bands (delta 2-4.5 Hz, theta 5-9 Hz, alpha 9-13 Hz, beta 13-20 Hz, low gamma 20-45 Hz, high gamma 53-80 Hz). For each band, 3 sub-columns report: background (pre-stimulation), beginning of ISI (2 s post-train offset), end of ISI (2 s pre-next train).

These files enable replication of population summaries, statistical tests, and frequency-specific coherence peaks used to design nested stimulation patterns.

Citation and Collaboration RequestIf you plan to publish research using this dataset, please cite the DOI and contact the dataset owner (email: [e-mail]) prior to submission. Please share your manuscript draft for review to ensure accurate representation of the data. Collaboration on publications is encouraged where the dataset forms a core component.