Real-time PCR analysis during mule duck development. Real-time PCR analysis during mule duck development

This study proposes to describe the basal hepatic metabolism at the level of mRNA in mule duck embryos A kinetic study was designed to determine the level of expression of selected genes involved in liver metabolism throughout embryogenesis Overall design: The livers of 20 mule duck embryos were collected every four days from the 12th day of embryogenesis (E12) until 4 days after hatching (D4), and gene expression analysis was performed. The expression levels of 50 mRNAs were quantified for these 7 sampling points and classified into 4 major cellular pathways