microRNA binding by wildtype Ago2 and mutant Ago2 in human cells
收藏NIAID Data Ecosystem2026-03-07 收录
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资源简介：
Ago2 binds mature microRNAs (miRNAs) to regulate gene expression. A mutant Ago2 is constructed to render it unable to bind miRNAs and inhibit mRNA translation. Human 293T cells were transiently transfected with a plasmid that encoded a FLAG-tagged wildtype human Ago2 or mutant Ago2. FLAG IP was preformed, and assoicated RNA was isolated and subjected miRNA microarray analysis.
创建时间：
2013-03-03
