Metagenomic evaluations of four samples of produced water from different Brazilian oil fields.

The negative impact of microbial action on oil platforms extends from extraction to the transportation and refining process. One of the biggest sources of contamination is the water produced, the main effluent from oil extraction. The water produced has in its composition high concentrations of sulfur salts, which make the environment ideal for the development of sulfur-reducing bacteria (BRE). BRE are responsible for the generation of hydrogen sulfide (H2S), an extremely toxic gas, and for the potentiation of corrosive effects in pipelines, tanks and equipment making the problem of great relevance. The most commonly used alternative in an attempt to resolve the issue is the use of high-dose commodity biocides, such as glutaraldehyde, tetrakis (hydroxymethyl) phosphonium (THPS) and 2,2-dibromo-3-nitrilepropionamide (DBNPA) . However, generalized treatments like these, in addition to offering a low cost / benefit, can act to boost the process of bacterial resistance, making control difficult. From these considerations, the objective of this work is to study the mechanisms of resistance to glutaraldehyde, THPS and DBNPA of BRE isolated from samples of water produced from Brazilian oil platforms. Using the techniques of New Generation Sequencing (NGS), Matrix Assisted Lazer Desorption Ionization (MALDI TOF) and Liquid Chromatography - tandem Mass Spectrometry (LC-MS / MS), microorganisms will be identified and proteomes will be evaluated for protein detection previously reported in the literature related to the bacterial resistance process. Through this study, it is expected to contribute to the elucidation of the process of resistance to biocides used in oil platforms, boosting the development and application of customized treatments, maximizing cost-benefit and minimizing environmental damage.