Role of TaVAP in stress tolerance in plants

Abiotic stresses like drought, salinity, high and low temperature, and submergence are major factors that limit the crop productivity. Hence, identification of genes associated with stress response in crops is a prerequisite for improving their tolerance to adverse environmental conditions. In this study, we have analyzed the expression profiles of three genotypes WT, TaVAP mutant and TaVAPOE plants in Arabidopsis thaliana in col-0 background using microarray technology to identify the genes differentially expressed under control conditions. We used microarrays to detail the global programme of gene expression underlying differential expression and identified distinct classes of up-regulated and downregulated genes during this process. Five-day-old light-grown Arabidopsis seedlings from three genotypes were grown under controlled conditions, which were further used for RNA extraction and hybridization on Affymetrix microarrays. Two biological replicates of each sample were used for microarray analysis.