Polyphasic study of phytopathogenic bacterial strains associated with deep bark canker of walnut in Serbia revealed a new species, Brenneria izbisi sp. nov.

In the present work, draft whole-genome sequences were obtained for two novel Brenneria strains (KBI 423T and KBI 447). De novo sequence assembly was performed using SPAdes 3.15.3 (with options -k 21,33,55,77 and --isolate) (Prjibelski et al., 2020). The genome sequences were annotated using Prokka (Galaxy Version 1.13) (Seemann, 2014). These files were used as an input for analyses performed in our study (core-genome phylogeny and OGRIs). Taken together, the dataset contained two strains studied (KBI 423T and KBI 447), as well as reference strains of Brenneria (14 strains), Dickeya (3 strains), Lonsdalea (3 strains) and Pectobacterium (3 strains). As an outgroup, we used members of the family Erwiniaceae, including Erwinia spp. (4 strains) and Pantoea spp. (4 strains). Here, we included raw reads used for de novo assembly of Brenneria genomes (KBI 423T and KBI 447). We also uploaded resulting assembly .fasta files, including those of reference strains used in analysis, as well as their annotation files obtained by prokka (.gbk files).