Digital sequencing using structured unique molecular identifiers

NIAID Data Ecosystem2026-05-02 收录

基因组测序技术优化

低频突变检测

数据链接：

https://www.ncbi.nlm.nih.gov/sra/SRP377776 数据链接链接失效反馈

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资源简介：

The aim of this study is to improve the performance of digital sequencing by SiMSen-seq by using structured unique molecular identifiers.

创建时间：

2025-02-11

Data from: Benefits and challenges with applying unique molecular identifiers in next generation sequencing to detect low frequency mutations

基因测序

低频突变检测

Indexing individual template molecules with a unique identifier (UID) before PCR and deep sequencing is promising for detecting low frequency mutations, as true mutations could be distinguished from P

DataONE2016-01-22 更新100

Influence of sequencing depth on the fidelity and sensitivity of 1%-5% low-frequency mutation detection

低频突变检测

测序深度优化

Influence of sequencing depth on the fidelity and sensitivity of 1%-5% low-frequency mutation detection and recommendations for standardization of sequencing depth calculation

DataCite Commons2021-12-27 更新60

Table_3_Bio-Layer Interferometry Analysis of the Target Binding Activity of CRISPR-Cas Effector Complexes.DOCX

CRISPR-Cas分子机制

基因组测序技术优化

CRISPR-Cas systems employ ribonucleoprotein complexes to identify nucleic acid targets with complementarity to bound CRISPR RNAs. Analyses of the high diversification of these effector complexes sugge

NIAID Data Ecosystem50

Application of a multiplexed ligase end-joining fidelity assay to optimize Golden Gate assembly

基因组测序技术优化

连接酶保真度分析

Using a comprehensive method for profiling end-joining ligation fidelity and bias to predict highly accurate sets of connections for ligation-based DNA assembly methods

NIAID Data Ecosystem80

Increase base editing efficiency and product variety through fusion of Cas9 nickase to cytosine and adenosine deaminases

基因组测序技术优化

碱基编辑器

Base editors, which are created through fusion of Cas9 nickase to either cytidine or adenine deaminase, efficiently catalyze site-specific nucleotide conversions with minimal indel rates. However, cyt

NIAID Data Ecosystem50

Digital sequencing using structured unique molecular identifiers

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