Topological analysis of the human beta 2-adrenergic receptor expressed in Escherichia coli.
收藏PubMed Central1994-10-25 更新2026-05-16 收录
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https://pmc.ncbi.nlm.nih.gov/articles/PMC45053/
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资源简介：
We have investigated the topology of the human beta 2-adrenergic receptor expressed in Escherichia coli, using the genetic method described by Beckwith and coworkers. We found that fusions with alkaline phosphatase beyond a certain point on the human beta 2-adrenergic receptor sequence were assembled into the bacterial membrane with the same topology as the human beta 2-adrenergic receptor in the mammalian membrane. The pattern that might have been expected on the basis of the topology of the human beta 2-adrenergic receptor in mammalian membranes was not reflected in the levels of alkaline phosphatase activity of the fusions occurring between the N-terminal region and positions close to the second external domain. Our data suggest that the correct positioning of the N terminus of the receptor depends on the presence of its C-terminal portions. IMAGES:
提供机构：
National Academy of Sciences
创建时间：
1994-10-25
