Next Generation Sequencing of Ezh2-cKO and Utx cKO transcriptomes of exostoses in mice

Ezh2-cKO or Utx-cKO in chondrocytes initiate metachondromatosis which containing both exostoses and enchondromas. To figure out whether the exostoses of Ezh2-cKO and Utx-cKO were the similar tumore, we compared the transcriptional profiles using RNA-sequencing of exostoses from Ezh2-cKO and Utx-cKO mice. Overall design: Examination of differential genes expression in 2 genotipic exostoses cells Exostoses in Ezh2-cKO or Utx-cKO-M mice at the 4 weeks after tumors were visible and the wild type articular cartilage from the same aged control mice were collected. RNA was extracted, purified, and checked for integrity using an Agilent Bioanalyzer 2100 (Agilent Technologies). Libraries were generated for sequencing using the TruSeq RNA sample preparation kit (Illumina). Libraries were sequenced using an Illumina HiSEq. 2500 sequencer. All of the above processes were performed at Shanghai Biotechnology Corporation, Shanghai, China