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Bulk RNA-seq of mouse cells from the dorsal cerebral cortex during its formation

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https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE134688
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Dorsal cortices from embryonic day (E10.5) to postnatal day 1 (PN) were micro-dissected and dissociated into single cell suspensions using Papain and Ovo-mucoid mix. Cells were washed with L15 medium and FAC-sorted for GFP positive NSCs using FACSariaIII (BD Biosciences) derived from Hes5::GFP transgenic embryos for NSCs and Tbr2::GFP transgenic embryos for BPs and NBNs. 70 samples of neural stem cells, basal progenitors and newborn neurons, daily from embryonic day 10.5 to first post-natal day.
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2023-01-11
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