Cytotoxicity of gentamicin and NB54 in HEK293 and HFF cells.

Aminoglycoside-induced cell toxicity was measured in human embryonic kidney cells (HEK293) and in human foreskin fibroblasts (HFF) treated for 48 h.aAminoglycosides are sulfate salts (MWgentamicin = 653.21 and MWNB54 = 652.81) and concentrations refer to the free amine form.bCell toxicity was calculated as ratio between the number of living cells in the presence of aminoglycoside versus non-treated cultures. The half-maximal lethal concentration (LC50) values were obtained from fitting concentration–response curves to the data obtained from at least three independent experiments, using GraFit 5 software.