High fat diet inhibits EV-Mediated angiogenisis

Mesenchymal stem cell-derived extracellular vesicles (EVs) have been shown to promote angiogenesis in the ischemic myocardium. This study examines the difference in vascular density, myocardial perfusion, molecular signaling, and gene expression between normal diet (ND) and high fat diet (HFD) groups at baseline and following intra-myocardial injection of EVs We used GeneChip Gene Arrays for whole-transcriptome analysis between normal diet and high fat diet groups at baseline and following intra-myocardial injection of Evs. Intact male Yorkshire swine fed either Normal diet (ND) or High fat diet (HFD) underwent placement of an ameroid constrictor on their left circumflex coronary artery. Subsequently ND and HFD animals received either intra-myocardial injection of vehicle-daline as controls or EVs into the ischemic territory. Five weeks later, myocardial tissue were harvest for RNA isolation and gene array.