European Zebrafish Resource Center

The EZRC at KIT houses the largest experimental fish facility in Europe with a capacity of more than 300,000 fish. Zebrafish stocks are maintained mostly as frozen sperm. Frequently requested lines are also kept alive as well as a selection of wildtype strains. Several thousand mutations in protein coding genes generated by TILLING in the Stemple lab of the Sanger Centre, Hinxton, UK and lines generated by ENU mutagenesis by the Nüsslein-Volhard lab in addition to transgenic lines and mutants generated by KIT groups or brought in through collaborations. We also accept submissions on an individual basis and ship fish upon request to PIs in Europe and elsewhere. EZRC also provides screening services and technologies such as imaging and high-throughput sequencing. Key areas include automation of embryo handling and automated image acquisition and processing. Our platform also involves the development of novel microscopy techniques (e.g. SPIM, DSLM, robotic macroscope) to permit high-resolution, real-time imaging in 4D. By association with the ComPlat platform, we can support also chemical screens and offer libraries with up to 20,000 compounds in total for external users. As another service to the community the EZRC provides plasmids (cDNAs, transgenes, Talen, Crispr/cas9) maintained by the Helmholtz repository of Bioparts (HERBI) to the scientific community. In addition the fish facility keeps a range of medaka stocks, maintained by the Loosli group.

德国卡尔斯鲁厄理工学院（KIT）的EZRC拥有欧洲最大的实验鱼类养殖设施，其容纳量超过30万尾鱼类。斑马鱼种群主要保存为冷冻精子。频繁请求的品系以及一系列野生型菌株亦被保持活性。此外，英国欣克斯顿Sanger中心Stemple实验室通过TILLING技术产生的数千个蛋白质编码基因突变体，以及Nüsslein-Volhard实验室通过ENU诱变产生的品系，以及KIT研究小组或通过合作引入的转基因品系和突变体也被保留。我们亦接受个别提交，并根据要求向欧洲及其他地区的负责人运送鱼类。EZRC还提供筛选服务和技术，如成像和高速测序。关键领域包括胚胎处理的自动化以及自动图像获取和处理。我们的平台还涉及新型显微镜技术的发展（例如，共聚焦显微镜成像、数字扫描显微镜、机器人显微镜），以实现四维的高分辨率实时成像。通过与ComPlat平台的关联，我们还能支持化学筛选，并向外部用户提供含有总计达20,000种化合物的库。作为对科学界的一项服务，EZRC还向科学界提供由生物零件赫尔姆霍兹仓库（HERBI）维护的质粒（cDNA、转基因、Talen、Crispr/cas9）。此外，鱼类养殖设施还保存了由Loosli小组维护的斑马鱼种群。