Sox6 expression distinguishes dorsally and ventrally biased dopamine neurons in the substantia nigra with distinctive properties and embryonic origins

To determine the molecular differences between ventral Sox6+ and dorsal Sox6- DA neurons, we performed bulk RNA-sequencing in sorted mCherry+ and GFP+ cells from the SNc of adult Sox6-FSF-Cre, Th-2A-Flpo, RC-Frepe mice. Our analysis revealed large and significant differential gene expression between these two populations. mRNA profiling of two dopaminergic populations (Sox6+ and Sox6-) in the SNc of adult mice brains.