Primary Data.zip
收藏DataCite Commons2025-11-04 更新2026-02-09 收录
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https://figshare.com/articles/dataset/Primary_Data_zip/30109795/3
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Cycloheptylprodigiosin exhibits potent anticancer activity through a unique mechanism involving the induction of severe Golgi stress, a previously unrecognized cell death pathway. To increase the prodigiosin production from the marine bacterium <i>Spartinivicinus ruber</i> MCCC 1K03745<sup>T</sup>, we investigated key fermentation parameters, including incubation time and initial medium pH. The culture medium composition was then sequentially optimized by single-factor experiments, a full factorial design, and an orthogonal design. Our results showed that the optimal incubation time was 30 h post inoculation, while initial pH had no effect on prodiginines production within the range of pH 6.0 to 8.0. By orthogonal design, the optimal medium was determined as follows: peptone 11 g/L, yeast extract 1 g/L, soybean oil 5 mL/L and MgCl<sub>2</sub>.6H<sub>2</sub>O 3 g/L in seawater. Verification experiments showed that prodiginine concentration under the optimized conditions reached 14.64 mg/L, representing 2.62 times the concentration obtained in basal Marine Broth 2216. These findings provide a basis for the cost-effective production of prodiginines from <i>S.</i><i> </i><i>ruber</i> MCCC 1K03745<sup>T</sup> for potential pharmaceutical applications.<br>
提供机构：
figshare
创建时间：
2025-11-04
