Perkinsus olseni ATCC:50984 transcriptome

The protozoan parasites Perkinsus spp. cause Dermo disease leading to mortalities of farmed and wild bivalve populations and hence have important detrimental economic and ecological consequences. We performed transcriptomic analysis of a Perkinsus olseni ATCC #50984 pure culture, grown in the absence of a host, to aid in the understanding of the parasite's biology. RNA was extracted from a culture in mid-exponential growth phase using standard Trizol purification. RNA was subjected to ribosomal RNA depletion prior to library preparation. Sequencing was carried out by Illumina HiSeq high output (125 bp PE).?