Root application of RNAi-induced gene silencing mechanism for protection of ash against emerald ash borer

In this study, we evaluate the systemic distribution and retention of exogenously applied dsRNA in green ash (Fraxinus pennsylvanica) tissues to investigate the feasibility of dsRNA delivery through the host plant. To evaluate the distribution and persistence of dsRNA, seedings are exposed to EAB-specific dsRNA as a root soak, sampled 3, 7, 14, 21, and 30d after exposure, and sectioned into root, woody-stem, soft-stem, and leaf tissues. Gel images and Sanger sequencing confirm the presence of exogenously applied dsRNAs, demonstrating successful uptake and translocation of dsRNAs throughout plant tissues.