Porcine Amygdala RNA-Sequencing

Prebreeding anestrus is a major reason for reproductive failure in gilts. The two types of prebreeding anestrus are delay in attaining puberty (prepubertal, PP) and silent estrus (behavioral anestrus, BA). Differential gene expression in the amygdala was analyzed in three comparisons; 1) PP cases and cyclic controls at follicular phase of estrous cycle, 2) BA cases and cyclic controls at luteal phase of estrous cycle, and 3) gilts at different stages of the ovarian cycle (cyclic gilts at follicular phase and luteal phase of estrous cycle) to understand gene expression profiles associated with different pubertal phenotypes and how the steroid hormone milieu in different stages of the estrous cycle affects gene expression in this key brain region.

预繁殖无发情期是母猪繁殖失败的主要原因。预繁殖无发情期可分为两种类型：达到青春期延迟（青春期前，PP）和沉默发情（行为性无发情，BA）。通过对杏仁核的差异基因表达进行了三次比较分析；1）青春期前病例与发情周期卵泡期的循环对照组，2）行为性无发情病例与发情周期黄体期的循环对照组，以及3）处于不同卵巢周期阶段的母猪（发情周期中的卵泡期和黄体期的循环母猪），以探究与不同青春期表型相关的基因表达谱，以及发情周期不同阶段中的类固醇激素环境如何影响这一关键脑区的基因表达。