Figure S1G -RelA-RNA complexes used for CRAC isolated before (-) and after (+) isoleucine starvation

RelA-RNA complexes used for CRAC isolated before and after isoleucine starvation from strains MG1655, MG1655relA::HTF, MG1655relAΔRRM::HTF, MG1655relAΔZFDΔRRM::HTF, MG1655relAH432E::HTF. The radiolabeled RelA-RNA complexes were separated by SDS-PAGE and transferred to a nitrocellulose membrane.

从菌株MG1655、MG1655relA::HTF、MG1655relAΔRRM::HTF、MG1655relAΔZFDΔRRM::HTF以及MG1655relAH432E::HTF中分离得到的用于CRAC的RelA-RNA复合体，在异亮氨酸饥饿前后进行提取。经过放射标记的RelA-RNA复合体通过SDS-PAGE进行分离，并转移至硝酸纤维素膜上。