BIOGRID CURATED DATA FOR PUBLICATION: Investigation of RNA metabolism through large-scale genetic interaction profiling in yeast.

Protein-Protein, Genetic, and Chemical Interactions for Decourty L (2021):Investigation of RNA metabolism through large-scale genetic interaction profiling in yeast. curated by BioGRID (https://thebiogrid.org); ABSTRACT: Gene deletion and gene expression alteration can lead to growth defects that are amplified or reduced when a second mutation is present in the same cells. We performed 154 genetic interaction mapping (GIM) screens with query mutants related with RNA metabolism and estimated the growth rates of about 700 000 double mutant Saccharomyces cerevisiae strains. The tested targets included the gene deletion collection and 900 strains in which essential genes were affected by mRNA destabilization (DAmP). To analyze the results, we developed RECAP, a strategy that validates genetic interaction profiles by comparison with gene co-citation frequency, and identified links between 1471 genes and 117 biological processes. In addition to these large-scale results, we validated both enhancement and suppression of slow growth measured for specific RNA-related pathways. Thus, negative genetic interactions identified a role for the OCA inositol polyphosphate hydrolase complex in mRNA translation initiation. By analysis of suppressors, we found that Puf4, a Pumilio family RNA binding protein, inhibits ribosomal protein Rpl9 function, by acting on a conserved UGUAcauUA motif located downstream the stop codon of the RPL9B mRNA. Altogether, the results and their analysis should represent a useful resource for discovery of gene function in yeast.

Protein-Protein, Genetic, and Chemical Interactions for Decourty L (2021)：通过酵母中大规模遗传互作分析探究RNA代谢。由BioGRID（https://thebiogrid.org）整理；摘要：基因敲除及基因表达变异可能导致细胞生长缺陷，当存在第二个突变时，这种缺陷会被放大或减弱。本研究对与RNA代谢相关的查询突变体进行了154次遗传互作映射（GIM）筛选，并估算约70万株双突变型酿酒酵母菌株的生长速率。测试的目标包括基因敲除集合和900株受mRNA不稳定性（DAmP）影响的必需基因菌株。为了分析结果，我们开发了RECAP策略，通过比较基因共引证频率来验证遗传互作图谱，并确定了1471个基因与117个生物过程之间的联系。除此之外，我们还验证了特定RNA相关通路中慢生长的增强和抑制现象。因此，负向遗传互作揭示了OCA肌醇多磷酸水解酶复合体在mRNA翻译起始中的作用。通过对抑制子的分析，我们发现Puf4，一种Pumilio家族RNA结合蛋白，通过作用于RPL9B mRNA下游的保守UGUAcauUA基序，抑制了核糖体蛋白Rpl9的功能。综上所述，这些结果及其分析应为酵母中基因功能发现提供一项有价值的资源。