Effect of the <i>Amr1</i> gene on lesion diameters (mm) following inoculation with wild-type <i>Alternaria brassicicola</i>, Δ<i>amr1:Amr1p-GFP</i>, Δ<i>amr1:Amr1</i>,and Δ<i>amr1:TrpCp-Amr1</i>.

Mean lesion diameters (mm) and their standard deviations in each column followed by the same letter were not significantly different based on the Waller-Duncan k-ratio (k = 100) t-test. * and ** indicate a significant effect of leaf age based on analysis of variance at p<0.05 and p<0.01, respectively. Increased virulence was calculated by the sum of lesion size differences between the wild type and mutant divided by the sum of wild-type lesion sizes multiplied by 100. combined = all data with lesions caused by wild type, Δamr1, Comp, and Cons mutants. Δamr1 = Δamr1:Amr1p-GFP, Compl = Δamr1:Amr1, Cons = Δamr1:TrpCp-Amr1.