Experimental (estimates) and calculated steady-state concentrations without logistic functions for each cellular state.

Concentrations for individual components are local and are given in units of μM. Data were estimated as described in Appendix C in S1 Text Simulated concentrations were within 1% of experimental values. The [Fecell] values listed are obtained by summing the concentrations of individual iron-containing component multiplied by the fractional volume (0.8 for cyt; 0.1 for vac; 0.1 for mit), [Fecell] = fcyt[CIA] + fvac[F2] + fvac[F3] + fcyt[FC] + fmit[FM] + fmit[FS] + fmit[MP]. For the first column, [Fecell] = 0.8⋅80 + 0.1⋅200 + 0.1⋅3400 + 0.8⋅20 + 0.1⋅100 + 0.1⋅500 + 0.1⋅50 = 505 μM. Comparable computed values as obtained by Fernandez et al. [20] are given on the right side of the table for comparison. Predictions for the HW and HY states assumed CRM case 1 autoregulation. Hw and HY state values were not used to train the model.