TipDC transcriptome.

At zero or nine days following intranasal infection of mice with IAV at (EID50: 2000,) the lungs were homogenized, and cells extracted. Cells were antibody stained for TipDC surface markers CD11b, Ly6c, GR-1, and MHCII. TipDC controls (Ctl) from day 0 were sorted based on high levels of CD11b, Ly6c, and GR-1. TipDC from day 9 of the IAV infection (IAV) were sorted based on high levels of CD11b, Ly6c, GR-1, and MHCII. cDNA libraries were generated from RNA, sequenced, and narrowed to confidently identified transcripts. (A) Transcripts differential expression was determined using Tukey’s HSD test for multiple comparisons with a Benjamini-Hochberg post hoc false discovery rate correction. (B) Transcripts that increased following IAV infection were submitted to DAVID, and significantly enriched KEGG pathways are listed with corresponding KEGG class and subclass from the BRITE hierarchy. (C) Transcripts from the KEGG metabolic classes were submitted to DAVID, and significantly enriched metabolic pathways are listed. (D) Transcripts that decreased following IAV infection were submitted to DAVID, and significantly enriched KEGG pathways are listed with corresponding KEGG class and subclass from the BRITE hierarchy. (E) Unique transcripts from the Immune Pathway and Influenza A pathway were differentially expressed, and corresponding protein names are listed under their functional category. (F) Gene symbols of the metabolic transcripts from TipDC and proteins from the in vitro differentiated DC are listed per pathway with the counts per cell type by downregulated (Down) or upregulated (Up) following IAV infection (G). (XLSX)