Fluorescence in situ hybridization (FISH) images of methanotrophic (MOX, MITC849 probe double-labelled with 594 dye) symbionts in 10 μm sections of I. methanophila sponge (Chapopote Knoll).
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We double-labelled MTC849 (5` – CGTTAGCTCCACCACTAAG – 3) with Atto594 dye (Biomers, Ulm, Germany), and hybridized it with sponge tissue in 20% formamide buffer using standard protocol (Duperron et al., 2008). Photomicrographs were acquired with confocal laser-scanning microscope (LSM 780, Carl Zeiss, Germany) - named CLSM*** or with Zeiss Axioplan 2 epifluorescence microscope (Zeiss, Jena, Germany) - named 7-108-ROV17*** . Three channels were acquired - 594, DAPI and 488/FITC (autofluoresence). Five images that had been acquired with 10x magnification have been used to reconstruct the overview representation.
创建时间：
2018-06-10
