Porcellionides pruinosus Transcriptome or Gene expression

Four total RNA-seq libraries were prepared from full organisms of P. pruinosus. One paired-end library that included equal amounts of total RNA extracted separately from organisms from different life stages: mancae, juveniles and adults (males and pregnant/non-pregnant females). Three other single-end libraries were also prepared corresponding to organisms in control treatments and exposed to nickel (50mg and 250mg/kg soil). Each of the single-end libraries was made by pooling equal amounts of total RNA extracted separately from three of the five exposed organisms whose RNA extractions showed the best quality.