GNPS - Methylobacter tundripaludum

Supernatant from a stationary phase culture of Methylobacter tundripaludum was run over a C18 solid phase extraction column. The column was washed with 10% methanol in water and then eluted with 90% methanol in water. The eluate was dried and run over a standard LC-MS/MS gradient (ACN in H2O with 0.1% formic acid) focusing on ions with a parent m/z of 150-600.

The excreted metabolome of the methane-oxidizing bacterium Methylobacter tundripaludum
21/22 and mutants related to the production of the natural product tundrenone. Supernatant from a stationary phase culture of Methylobacter tundripaludum 21/22 was extracted twice with an equal volume of ethyl acetate containing 0.01% acetic acid. The organic extract was then dried and analyzed over a standard LC-MS/MS gradient (ACN in H2O with 0.1% formic acid). mbaI: Quorum sensing deficient mutant. mbaIplusSig: Quorum sensing deficient mutant grown in the presence of 1 uM exogenous quorum sensing signal 3-OH-C10-HSL. tunJ: Tundrenone deficient mutant lacking the annotated acyl-CoA ligase TunJ.