Loss of infectivity by direct physical interaction of SwIV and E. faecium.
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After preincubation of SwIV and E. faecium for 1.5 h, the mixture was centrifuged and supernatants were transferred onto monolayers of 3D4/21 and MDBK cells to determine the virus titers in the original supernatants by TCID50 at 48 hpi or 96 hpi. Aliquots from the growth medium of the virus titration were taken to determine NO release. As a control, SwIV was preincubated without adding any E. faecium and the samples processed in parallel to the ones with the probiotic. Results are means ± standard deviations from three independent experiments.**P
创建时间：
2015-12-02
