Metatranscriptomics of Salmonella superspeader stool. Metatranscriptomics of Salmonella superspeader stool

Longitudinal analysis of Salmonella typhimurium mRNA from superspeader mouse cecal content and stool compared to in vitro Salmonella typhimurium mRNA. Overall design: Mice were infected with 10^8 colony forming units of Salmonella Typhimurium. 3 mice were euthanized at day 14 and 21 post-infection. Cecal content and feces were collected. RNA was extracted from those cecal contents and feces. In addition, salmonella was grown in LB for 10-16 hours aerobically and anaerobically for RNA extraction. In vivo and in vitro samples were sequenced and differential expression analysis was performed comparing the two sample groups.