Analysis of mouse antigen specific germinal centre B cell subset gene signatures in presence and absence of kidney disease during T dependent immunization

Wild type or Aristolochic acid (AAI) nephropathy (AAN) mice were immunized with NP-KLH in Alum (100ug/mouse) On day 12 post-immunization, live NP-GcB cells were FACS-sorted from spleen according to B220+ GL7+ CD95+ NP+ expression directly into SmartSeq low-input RNA kit lysis buffer Overall design: Intra-peritoneal AAI (7.5mg/kg body wt) induce AAN, on day 4 of AAN, mouse immunized with NP-KLH, on day 12 post-immunization, mice sacrificed for spleen harvest