Additional file 5 of Pathological mechanisms and candidate therapeutic approaches in the hearing loss of mice carrying human MIR96 mutations

Additional file 5:  Table S4. Gene sets enriched in up- and downregulated genes in Mir96 +13G>A and Mir96 +14C>A homozygotes. For each gene set, the number of the genes present in the RNA-seq dataset is shown, along with enrichment score (which indicates the degree of overrepresentation of the gene set at the top or the bottom of the list of input genes ranked by differential expression), normalised enrichment score (which accounts for gene set size), p value (significance of enrichment score for the single gene set) and false discovery rate (FDR; the probability that a gene set with that normalised enrichment score represents a false positive finding, corrected for multiple testing and gene set size). Gene sets are ordered by FDR. Two gene sets corresponding to predicted miR-96 targets are enriched in upregulated genes in the Mir96  +  13G  >  A transcriptome, but neither are enriched in the Mir96  +  14C  >  A transcriptome, which means that the genes in those gene sets are relatively evenly distributed through the Mir96  +  14C  >  A RNA-seq gene list when ranked by differential expression. This could be due to the much greater number of significantly misregulated genes in the Mir96 +14C>A transcriptome, which may mask the signal from the loss of the wildtype miR-96 targeting.