Transcriptomics in human cumulus cells during oocyte in vitro maturation

Advancing human oocyte in vitro maturation (IVM) requires understanding the mechanisms that govern this process. The cumulus cells (CCs) that surround the oocyte play a crucial role. Our study focused on identifying specific genes in human CCs contributing to oocyte maturation in vitro.
We used microarrays to detail the global transcriptome underlying oocyte maturation in cumulus cells and identified distinct up-and-down-regulated genes and signaling pathways during this process. Cumulus cells were removed from immatures (germinal vesicle - GV) cumulus-oocyte complexes (COCs) before IVM (Fresh GV, n = 5) and immature (GV) and mature (MII) COCs after IVM (GV-IVM, n=8; and MII-IVM, n=12). All the samples were collected ex vivo from 8 women who underwent unilateral ovariectomy and ovarian tissue cryopreservation for fertility preservation. Women did not receive prior ovarian stimulation, Total RNA was individually extracted and purified from each sample with TRIzol® reagent and 1-bromo-3-chloropropane and, subsequently, with RNeasy® Minikit 250 (Qiagen, Denmark) according to the manufacturer’s instructions. All steps were performed on ice. RNA quality and quantity were assessed by DeNovix DS-11 FX spectrophotometer and Bioanalyzer RNA 6000 Pico Kit, respectively. Only samples with RNA integrity (RIN) values ≥ 6 were included in the study. Moreover, 25 samples were selected based on their RIN values to ensure a similar distribution across all groups: Fresh GV (mean RIN 8.9, n = 5), GV (mean RIN 7.9, n = 8), and MII (mean RIN 8, n = 12) (Supplementary Table S2). The selected samples were further processed using the ClariomTM D arrays with GeneChipTM Whole Transcript (WT) PLUS Reagent Kit according to the manufacturer’s instructions. The array was washed and stained with phycoerythrin-conjugated streptavidin using the Affymetrix Fluidics Station 450 and then scanned with t..., , # Transcriptomics in human cumulus cells during oocyte in vitro maturation

[https://doi.org/10.5061/dryad.66t1g1k9d](https://doi.org/10.5061/dryad.66t1g1k9d)

## Description of the data and file structure

Advancing human oocyte in vitro maturation (IVM) requires understanding the mechanisms that govern this process. The cumulus cells (CCs) surrounding the oocyte play a crucial role. Our study focused on identifying specific genes in human CCs that may contribute to oocyte maturation in vitro.

Microarray (ClariomTM D arrays with GeneChipTM Whole Transcript (WT) PLUS Reagent Kit) was used to evaluate the overall gene expression of CCs from fresh immature (germinal vesicle - GV) human oocytes (Fresh-GV) and CCs from immature (GV) and mature (metaphase II - MII) oocytes after in vitro maturation (GV-IVM and MII-IVM, respectively).

All the samples were collected ex vivo from the surplus ovarian medulla tissue of 8 women who underwent unilateral ovariectomy and ovarian tissue cryopreserva..., All patients provided explicit informed consent to donate their surplus ovarian tissue for research purposes, including the potential publication of de-identified data in the public domain.
The data shared in this submission has been fully de-identified. No personal identifiers (e.g., names, social security numbers, etc.) were collected or accessed. Patients were assigned random internal identification numbers that cannot be traced back to individuals. The only other available information includes diagnosis, which is not sufficient to identify any individual participant.