miRNAs expression in swine monocytes stimulated with Bifidobacterium animalis ssp. lactis Bb12

Bifidobacterium animalis ssp. lactis Bb12 (Bb12) is a probiotic bacteria with several reported benefits, including immunomodulatory capacity. This is due to the bacteria recognition by receptors such as TLR2, which in turn affects the miRNA transcriptome of immune system cells. We aimed to analyze miRNA expression in swine monocytes stimulated with Bb12, and their expression when TLR2 receptor is blocked. A microarray containing 407 porcine unique miRNA probes was employed to identify differences in the miRNA expression pattern in swine monocytes in response to Bb12. A total of 143 were differentially expressed miRNAs (p<0.05); out of which, 87 miRNAs are statistically significant but have lower signals (signal <500). Swine monocytes from peripheral blood were stimulated with Bifidobacterium animalis ssp. lactis Bb12 for 4 hours, with and without anti-TLR2 blocking antibody. Biological replicates: 1; Array replicates: 8.