Transcriptional profiling of cochlear neurons

In order to characterize the various subpopulations of neurons present in the inner ear, we dissected out the cochleas from wild-type mice, removing non-neuronal populations as possible, then dissociated the tissue to obtain a single cell suspension and profiled these by single cell RNA-Seq. This study's goal was to characterize the native populations of murine cochlear neurons. To ensure reproducibility of our findings, neurons from 4 separate mice were profiled.