Role of SecDF in virulence against Galleria mellonella insect larvae.

aInfections with mid log phase (OD600nm = 1) vegetative bacteria cultured in LB medium.bFor oral infection the bacteria are mixed with 3 µg/10 µl of Cry1C toxin. The toxin alone results in ≈10% mortality.cLC50, correspond to the bacterial dose (cfu, colony forming units) per larvae, killing 50% of the treated larvae.dconfidence interval at 95% level.*(P-value ≤0.05). LDL (lower dose limit), UDL (upper dose limit). Mortality was estimated by Probit analysis (StatPlus). based on at least two independent experiments. Control experiments were run with buffer (PBS) and no mortality occurred within 72 hours at 37°C.