Short and long read sequencing of human mammary epithelial MCF10a-Snail-ER cells after Epithelial-to-Mesenchymal Transition initiation

We cultured MCF10a-Snail-ER cells and induced EMT initiation with tamoxifen. A matched sequencing of their PolyA RNA was performed, using Illumina and direct RNA Oxford Nanopore sequencing technologies. Both generated datasets supported the development of hybrid bioinformatics tools. Parallel sequencing of matched MCF10a-Snail-ER PolyA RNA samples using Illumina and direct RNA Oxford Nanopore technologies.