The influence of melanoma extracellular vesicles on benign melanocytes, dermal fibroblasts and microvascular endothelial cells (part1)

This study investigates the role of PRAME (Preferentially Expressed Antigen in Melanoma), a cancer-testis antigen, in melanoma progression, particularly its function in melanoma-derived extracellular vesicles (EVs) and its impact on benign primary melanocytes (PM), primary fibroblasts(PF) and microvascular endothelial cells (EN). To investigate the effects of extracellular vesicles from melanoma cells on neighboring benign cells, we treated PM, PF, and EN cell lines with melanoma-derived conditioned medium from A375 cells. We then extracted total RNA and performed RNA sequencing.