Dietary metabarcoding datasets for the southern meadow jumping mouse (Zapus luteus luteus)

The southern meadow jumping mouse (Zapus luteus luteus) is Endangered in
the US due to loss of riparian habitat and knowledge of diet can focus
conservation efforts. Context and life stages of diet have been documented
for other species of Zapus (seeds, insect larvae, and hypogeous fungi),
possibly shifting toward greater diversity in seed predations prior to
hibernation. Yet, challenges in field observation, thorough digestion, and
lack of scalability have hindered a thorough survey of dietary taxonomies.
We collected the feces of 165 Z. l. luteus from across the geographic
range. Emphasizing granivorous diet, we used DNA metabarcoding to resolve
its dietary taxonomies. With negligible impact of field contamination, we
found a varied diet most frequently of graminoids, forbs, and lepidoptera.
Detecting up to nine dietary taxa in individuals, Z. l. luteus consumed
more and different combinations of plant taxa as they approached the known
hibernation window. Our work emphasizes the importance of forbs and
graminoids as the foundation of the diet, providing primary sustenance and
habitat for insect prey. The breadth of different diet items they consume
suggests plasticity in resource use, potentially accommodating diverse
patterns of seed production throughout their active period. We also
hypothesize that a peak in seed availability in the late season could play
a role in their accumulation of pre-hibernation fat stores.