Kinetic parameters of L-arginine transport.
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HUVECs were coincubated (8 hours) in the absence (−NBTI) or presence (+NBTI) of 10 µmol/L nitrobenzylthioinosine (NBTI), without (Control) or with insulin (1 nmol/L), ZM-241385 (10 nmol/L) and/or CGS-21680 (30 nmol/L) (see Methods). Maximal velocity (Vmax) and apparent Michaelis-Menten constant (Km) of saturable transport were calculated assuming a single Michaelis-Menten hyperbola. *P
创建时间：
2015-12-02
