Analgesic targets identified in mouse sensory neuron somata and terminal pain translatomes

The relationship between transcription and protein expression is complex. We identified polysome-associated RNA transcripts in the somata and central terminals of mouse sensory neurons in control, painful (+NGF) and pain-free conditions (Nav1.7 null mice). The majority (98%) of translated transcripts are shared between male and female mice, as are transcripts in the somata and terminals. However, some transcripts are enriched in the somata or terminals. Changes in the translatome in painful and pain-free conditions include novel and known regulators of pain pathways. Antisense knockdown of selected somatic and terminal polysome-associated transcripts that correlate with pain states diminished pain behaviour. Terminal-enriched transcripts encoding synaptic proteins, non-coding RNAs, transcription factors, proteins associated with trans-synaptic trafficking, GABA-generating enzymes and neuropeptides suggest that central terminal translation is a significant regulatory locus for peripheral input from sensory neurons.
TRAP gene expression and samples annotation files
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