Analysis of global gene expressions in initial infection of Mycobacterium avium subsp. paratuberculosis using epithelial cells-peripheral blood mononuclear cells culture passage model.

Purpose: Understanding host-MAP interactions in initial stage of infection Methods: Native MAP and MDBK processed (4h) MAP were infected to bovine PBMCs. Total RNA after 24h and 72h were harvested. RNA-Seq was performed using total RNA of each group. Result: The gene expression patterns commonly activated in both groups suggest that Th17 activation is the main immunological response to MAP infection. Overall design: in vitro epithelial cells-peripheral blood mononuclear cells (PBMCs) culture passage model to establish a host-MAP interaction during initial stage of infection.