In fission yeast, 65 non-essential mitochondrial proteins related to respiration and stress become essential in low-glucose conditions

Mitochondria perform critical functions, including respiration, ATP production, small molecule metabolism, anti-oxidation, and they are involved in a number of human diseases. While the mitochondrial genome contains a small number of protein-coding genes, the vast majority of mitochondrial proteins are encoded by nuclear genes. In fission yeast, Schizosaccharomyces pombe, we screened 457 deletion (del) mutants deficient in nuclear-encoded mitochondrial proteins, searching for those that fail to form colonies in culture medium containing low glucose (0.03–0.1%; low-glucose sensitive, lgs), but that proliferate in regular 2–3% glucose medium. Sixty-five (14%) of the 457 deletion mutants displayed the lgs phenotype. Thirty-three of them are defective either in dehydrogenases, subunits of respiratory complexes, the TCA cycle, or in one of the 9 steps of the CoQ10 biosynthetic pathway. The remaining 32 lgs mutants do not seem to be directly related to respiration. Fifteen are implicated in t..., , , # In fission yeast, 65 non-essential mitochondrial proteins related to respiration and stress become essential in low-glucose conditions [https://doi.org/10.5061/dryad.4j0zpc8fw](https://doi.org/10.5061/dryad.4j0zpc8fw) These files include electronic supplementary materials for the article \"In fission yeast, 65 non-essential mitochondrial proteins related to respiration and stress become essential in low-glucose conditions.\" In fission yeast, Schizosaccharomyces pombe, we screened 457 deletion mutants deficient in nuclear-encoded mitochondrial proteins, searching for those that fail to form colonies in culture medium containing low glucose (0.03-0.1%, low glucose sensitive, lgs), but that proliferate in regular 2-3% glucose medium. This dataset contains a list of deletion mutants screened in this study (Supplementary Table S2), a detailed list of genes required for cell proliferation in low glucose (Supplementary Table S1), and cell proliferation of the 10 selected lgs mutant strains...