Repeated biocide treatment of a Biofilm Model Based on Metagenomic Characterization of Drains in Seafood and Dairy Processing Facilities. Repeated biocide treatment of a Biofilm Model Based on Metagenomic Characterization of Drains in Seafood and Dairy Processing Facilities

Mock biofilm drain models that reflect the microbiota in floor drains in industrial food processing environments are needed to understand and optimize sanitation schemes. The purpose of this study was to develop a mock drain biofilm model suitable for studies investigating the efficacy of biocides. Eleven stainless steel floor drains in different food industries (shrimp, n=4; fish, n=4; dairy, n=3) were sampled. Dairy drains were sampled twice, nine months apart. The present study developed a biofilm model that reflects the microbiota in industrial floor drains and can be used to test the efficacy of biocides under simulated industrial conditions. Results from this research will contribute to the design and optimization of current and new sanitation schemes.