High-resolution spatial RNA profiling of tissues with immuno-labeling based LCM
收藏NIAID Data Ecosystem2026-03-12 收录
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资源简介：
We report a robust protocol to effectively maintain high RNA quality for immuno-fluorescence-based laser capture microdissection (LCM), enabling finely resolved spatial transcriptome acquisition with only tens of cells from snap-frozen or RNAlater-treated tissues. With the repertoire of readily-available antibodies, this method fulfils the potential of LCM to determine native transcriptomic landscapes, as exemplified here with the characterization of mouse lacteal tip and tube cells.
创建时间：
2021-09-30
