Gene expression profiles of rat hypertrophic zone after treatment of CNP53 or U0126 [U0126]

To investigate the similarity of gene expression profile of CNP53 and U0126 treatment in growth plate. Tibiae from E18.5 embryonic rat were isolated and pre-cultured in BGJb media for 1 day, followed by culturing in the presence of vehicle or U0126 (0.02mM) for 1 day. The tibiae (12 bones) were micro-dissected into the hypertrophic zone and pooled, from which RNA was isolated by using Qiagen RNeasy micro Kit, then microarray analyses were performed by using Agilent array system.