In Vivo CRISPR Screens Identify E3 Ligase Cop1 as a Modulator of Macrophage Infiltration and Cancer Immunotherapy Target

Cop1 regulates stability of transcription factors. We found that Cop1 can regulate the proteasomal degradation of Cebpd. We generated 4T1 breast cancer cells with CRISPR/Cas9 mediated Cop1 (gene symbol RFWD2) knockout. We used Cebpd antibody to pulldown the DNA fragments binding with Cebpd and did ChIP-seq. Examination of Cebpd ChIP-seq under two condtions in 4T1 breast cancer cells: Rosa26 and Cop1 knockout. Additionally, we used two different gRNAs to knockout Cop1. Two replicaters per condition.