Gene expression signatures in FAM46C (TENT5C)-deleted multiple myeloma (MM) cell lines

To investigate the effect of FAM46C disruption on gene expression, we generated FAM46C-knockout (FAM46C-KO) cell clones using CRISPR/Cas9 system with MM cell lines OCI-My5, KMS-11, and ANBL-6. Using the generated cell clones, we have employed whole genome microarray expression profiling as a discovery platform to identify genes that are associated with FAM46C expression in MM cell lines. OCI-My5, KMS-11, and ANBL-6 cell clones were cultured for 48 h in vitro. OCI-My5 and KMS-11 cells were maintained in RPMI 1640 supplemented with 10% FBS, while the IL-6-dependent ANBL-6 cells were maintained in RPMI1640 medium supplemented with 10% FBS in the presence of recombinant human IL-6 (10 ng/mL; R&D systems, Minneapolis, MN, USA) at 37 °C in 5% CO2 humidified atmosphere.