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Long-Term Cell Fate and Functional Maintenance of Human Hepatocyte through Stepwise Culture Configuration

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https://www.ncbi.nlm.nih.gov/sra/SRP385878
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Humanized Liver Chimeric Mice (HLCM)-derived human hepatocytes (HLCM-HH) were maintained in the absence (non-treatment; NT) or presence of either 2% DMSO or DMSO2 (140mM)-containing medium for 7 days. HepG2 cells and Huh7 cells were cultured with 2% DMSO containing medium for 7 days. Then total cellular RNA was extracted for the subsequent poly-A based mRNA sequencing analysis. Overall design: Freshly isolated Humanized Liver Chimeric Mice (HLCM)-derived human hepatocytes (HLCM-HH) cultured with 2% DMSO containing media for 7 days were further maintained in the absence (non-treatment; NT) or presence of either 2% DMSO or DMSO2 (140mM)-containing medium for an additional 7 days. HepG2 cells and Huh7 cells were cultured with 2% DMSO containing medium for 7 days. Then total cellular RNA was extracted for the subsequent poly-A based mRNA sequencing analysis.
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2023-01-13
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