Maternal/fetal chorioamnion tissue inflammatory responses for pregnant ewes colonized intra-amniotically with ureaplasmas for 3 days, 7 days or 69 days compared to the responses within control animal chorioamnion tissues.

1maternal inflammatory responses as stage 1 (early; acute subchorionitis or chorionitis), stage 2 (intermediate; acute chorioamnionitis), and stage 3 (advanced; necrotizing chorioamnionitis), Grade 1: mild-moderate; (a) with 1+ macrophages within the chorion; (b) with 2+ macrophages and ≥1+ neutrophils within the chorion; Grade 2: severe. 2fetal inflammatory responses as stage 1 (early; chorionic vasculitis or umbilical phlebitis), stage 2 (intermediate; umbilical vasculitis, one or two arteries, and/or vein or umbilical panvasculitis, all vessels), and stage 3 (advanced; [subacute] necrotizing funisitis or concentric umbilical perivasculitis). Grade 0: nil; 1: mild-moderate; Grade 2: severe. (Redline et al. 2003). 3Control animals injected with media intra-amniotically: 3d (n = 1), 7d (n = 2) and 69d (n = 4) prior to surgical delivery. 4a 69d control animal.