Evalutation of DEGs by RNA-seq in panobinostat-exposed (5 mg/kg/day) embryonic murine ovary

We hypothesized that gestational exposure (E6.5-E15.5) to panobinostat, a HDAC inhibitor, can lead to alteration of murine ovary transcriptome. To test our hypothesis, we exposed mice (C57Bl6j) to 5 mg/kg/day of panobinostat during embryonic development (E5.5-E15.5). Four embryonic ovaries were pooled prior RNA extraction using RNeasy Plus kit. RNA libraries (3 control, 3 exposed) were prepared using the NEBNext Ultra II Directional RNA Library Kit for Illumina. After pre-processing of the sequencing data we performed an analysis of differentially expressed genes. For each replicate, RNA was extracted from the pool of 6 to 8 embryonic ovaries (E15.5). Three biological replicates were prepared for each condition. Stranded libraries were prepared using NEBNext Ultra II Directional RNA Library Prep Kit for Illumina according manufacturer's instructions. Ov_RNA_Con1 : Library for exposed mouse 2 Ov_RNA_Con2 : Library for exposed mouse 3 Ov_RNA_Con3 : Library for exposed mouse 4 Ov_RNA_PB1 : Library for exposed mouse 2 Ov_RNA_PB2 : Library for exposed mouse 3 Ov_RNA_PB3 : Library for exposed mouse 4