Use of Human Adult Stem Cell-Derived Organoid Cultures to Model Enterohepatic Circulation

Organ to organ interactions have not yet been modeled with human organoid cultures. This work demonstrates the coupling of intestinal and liver organoids recapitulates aspects of enterohepatic bile signaling. The integration of organoids can be used to probe intra-organ interactions. Single cell RNA-Seq studies were performed in human liver organoids to examine the heterogeneity of the cultures. Liver organoids from three independent individuals were used for single-cell RNA-sequencing (scRNA-Seq).